Light-activated mitochondrial fission through optogenetic control of mitochondria-lysosome contacts.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35879298.
- Also identified by DOI 10.1038/s41467-022-31970-5 and PMC identifier 9314359.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Mitochondria are highly dynamic organelles whose fragmentation by fission is critical to their functional integrity and cellular homeostasis. Here, we develop a method via optogenetic control of mitochondria-lysosome contacts (MLCs) to induce mitochondrial fission with spatiotemporal accuracy. MLCs can be achieved by blue-light-induced association of mitochondria and lysosomes through various photoactivatable dimerizers. Real-time optogenetic induction of mitochondrial fission is tracked in living cells to measure the fission rate. The optogenetic method partially restores the mitochondrial functions of SLC25A46<sup>-/-</sup> cells, which display defects in mitochondrial fission and hyperfused mitochondria. The optogenetic MLCs system thus provides a platform for studying mitochondrial fission and treating mitochondrial diseases.
Medical subject headings
- Mitochondrial Diseases
- Mitochondrial Dynamics