Multiplex base editing to convert TAG into TAA codons in the human genome.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35918324.
- Also identified by DOI 10.1038/s41467-022-31927-8 and PMC identifier 9345975.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and examine base-editing genome-wide (observing ~40 C-to-T off-target events in essential gene exons). We also introduce GRIT, a computational tool for recoding. This demonstrates the feasibility of recoding, and highly multiplex editing in mammalian cells.
Medical subject headings
- Gene Editing
- Genome, Human