ntHash2: recursive spaced seed hashing for nucleotide sequences.

Kazemi, Parham; Wong, Johnathan; Nikolić, Vladimir; Mohamadi, Hamid; Warren, René L; Birol, Inanç · Bioinformatics · 2022

basic_science · Level V

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Abstract

Spaced seeds are robust alternatives to k-mers in analyzing nucleotide sequences with high base mismatch rates. Hashing is also crucial for efficiently storing abundant sequence data. Here, we introduce ntHash2, a fast algorithm for spaced seed hashing that can be integrated into various bioinformatics tools for efficient sequence analysis with applications in genome research. ntHash2 is up to 2.1× faster at hashing various spaced seeds than the previous version and 3.8× faster than conventional hashing algorithms with naïve adaptation. Additionally, we reduced the collision rate of ntHash for longer k-mer lengths and improved the uniformity of the hash distribution by modifying the canonical hashing mechanism. ntHash2 is freely available online at github.com/bcgsc/ntHash under an MIT license. Supplementary data are available at Bioinformatics online.

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