Spatially patterned hydrogen peroxide orchestrates stomatal development in Arabidopsis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36028510.
- Also identified by DOI 10.1038/s41467-022-32770-7 and PMC identifier 9418256.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Stomatal pores allow gas exchange between plant and atmosphere. Stomatal development is regulated by multiple intrinsic developmental and environmental signals. Here, we show that spatially patterned hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays an essential role in stomatal development. H<sub>2</sub>O<sub>2</sub> is remarkably enriched in meristemoids, which is established by spatial expression patterns of H<sub>2</sub>O<sub>2</sub>-scavenging enzyme CAT2 and APX1. SPEECHLESS (SPCH), a master regulator of stomatal development, directly binds to the promoters of CAT2 and APX1 to repress their expression in meristemoid cells. Mutations in CAT2 or APX1 result in an increased stomatal index. Ectopic expression of CAT2 driven by SPCH promoter significantly inhibits the stomatal development. Furthermore, H<sub>2</sub>O<sub>2</sub> activates the energy sensor SnRK1 by inducing the nuclear localization of the catalytic α-subunit KIN10, which stabilizes SPCH to promote stomatal development. Overall, these results demonstrate that the spatial pattern of H<sub>2</sub>O<sub>2</sub> in epidermal leaves is critical for the optimal stomatal development in Arabidopsis.
Medical subject headings
- Arabidopsis
- Arabidopsis Proteins