Enzymatic blood group conversion of human kidneys during ex vivo normothermic machine perfusion.

MacMillan, Serena; Hosgood, Sarah A; Nicholson, Michael L · Br J Surg · 2023

basic_science · Level V

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Abstract

A major restriction to transplantation is the requirement for ABO blood group compatibility between donor and recipient. In this study, an α-galactosidase enzyme from <i>Bacteroides fragilis</i> was used successfully to remove type B blood group antigens enzymatically from human kidneys using <i>ex vivo</i> normothermic machine perfusion. This provides the first step for a strategy to overcome the ABO barrier in kidney transplantation.

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