DNA-PAINT MINFLUX nanoscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36050490.
- Also identified by DOI 10.1038/s41592-022-01577-1 and PMC identifier 9467913.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on-off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells.
Medical subject headings
- DNA
- Fluorescent Dyes