RNA velocity unraveled.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36094956.
- Also identified by DOI 10.1371/journal.pcbi.1010492 and PMC identifier 9499228.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We perform a thorough analysis of RNA velocity methods, with a view towards understanding the suitability of the various assumptions underlying popular implementations. In addition to providing a self-contained exposition of the underlying mathematics, we undertake simulations and perform controlled experiments on biological datasets to assess workflow sensitivity to parameter choices and underlying biology. Finally, we argue for a more rigorous approach to RNA velocity, and present a framework for Markovian analysis that points to directions for improvement and mitigation of current problems.
Medical subject headings
- RNA