Evaluation of antibacterial effect of concentrated growth factor on <i>Aggregatibacter actinomycetemcomitans</i> and <i>Porphyromonas gingivalis</i>.

Abstract

<i>Aggregatibacter actinomycetemcomitans</i> and <i>Porphyromonas gingivalis</i> are the two main bacteria that cause periodontitis which is an inflammatory disease of periodontal tissues. Numerous antibacterial agents have been introduced to fight against these periodontal pathogens, but the antibacterial efficacy of concentrated growth factor has not been studied yet. Therefore, this study is aimed to investigate the antibacterial impact of concentrated growth factor on the growth of <i>Aggregatibacter actinomycetemcomitans</i> and <i>Porphyromonas gingivalis</i>. In this study, concentrated growth factor was obtained from blood samples of healthy people with no systemic disease and no antibiotics used for at least 3 months before the experiments. The concentrated growth factor from each blood sample was divided into two parts, i.e., three samples in <i>P. gingivalis</i> group and three samples in A. actinomycetemcomitans group, and a positive control group to verify the accuracy of the procedure and a negative group to rule out any contamination. The minimum inhibitory concentration and minimum bactericidal concentration of CGF against <i>A. actinomycetemcomitans</i> and <i>P. gingivalis</i> were determined by the broth microdilution method. Statistical analysis was performed using SPSS 22 software, and <i>P</i> value significance was set to 0.05. The antibacterial property of CGF on the studied bacteria in concentrations of MIC and ¼ MIC is the same for both bacteria, but in ½ MIC, its impact on <i>A. actinomycetemcomitans</i> is significantly higher than <i>P. gingivalis</i>. Examining the MBC showed that no MBC dose was obtained. Results of the disk diffusion test indicated the lack of the zones of bacterial growth inhibition. Considering the limitations of our study, it can be concluded that CGF has antibacterial effectiveness against <i>A. actinomycetemcomitans</i> and <i>P. gingivalis</i> with no significant difference between two bacteria.