Engineered CRISPR prime editors with compact, untethered reverse transcriptases.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36163548.
- Also identified by DOI 10.1038/s41587-022-01473-1 and PMC identifier 10023297.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as efficiently as intact PE2 in human cells. We use this Split-PE system to rapidly identify and engineer more compact prime editor architectures that also broaden the types of RTs used for prime editing.
Medical subject headings
- CRISPR-Cas Systems
- Gene Editing
- Moloney murine leukemia virus
- RNA-Directed DNA Polymerase
- Streptococcus pyogenes