A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36163549.
- Also identified by DOI 10.1038/s41587-022-01444-6 and PMC identifier 9931575.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Here we describe a competitive genome editing method that measures the effect of mutations on molecular functions, based on precision CRISPR editing using template libraries with either the original or altered sequence, and a sequence tag, enabling direct comparison between original and mutated cells. Using the example of the MYC oncogene, we identify important transcriptional targets and show that E-box mutations at MYC target gene promoters reduce cellular fitness.
Medical subject headings
- Gene Editing
- Transcription Factors