Advances in nanopore direct RNA sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36203024.
- Also identified by DOI 10.1038/s41592-022-01633-w and PMC identifier 11388133.
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Abstract
Nanopore direct RNA sequencing (DRS) reads continuous native RNA strands. Early adopters have used this technology to document nucleotide modifications and 3′ polyadenosine tails on RNA strands without added chemistry steps. Individual strands ranging in length from 70 to 26,000 nucleotides have been sequenced. In our opinion, broader acceptance of nanopore DRS by molecular biologists and cell biologists will be accelerated by higher basecall accuracy and lower RNA input requirements.
Medical subject headings
- Nanopore Sequencing
- Nanopores