Pervasive donor DNA integration defies precision gene editing of hematopoietic stem cells.
editorial · Level V
Where this comes from
- Record sourced from PubMed, PMID 36206729.
- Also identified by DOI 10.1016/j.stem.2022.09.003.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Therapeutic gene editing based on homology-directed repair (HDR) promises to precisely recombine donor template sequences at programmed double-strand break sites. In this issue, Ferrari et al. report that commonly used AAV donors blunt hematopoietic repopulation and result in frequent genomic integrations that could interfere with normal gene expression.
Medical subject headings
- DNA Breaks, Double-Stranded
- Gene Editing