Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36302022.
- Also identified by DOI 10.1126/science.abm0646.
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Abstract
To synthesize a chirally inverted ribosome with the goal of building mirror-image biology systems requires the preparation of kilobase-long mirror-image ribosomal RNAs that make up the structural and catalytic core and about two-thirds of the molecular mass of the mirror-image ribosome. Here, we chemically synthesized a 100-kilodalton mirror-image T7 RNA polymerase, which enabled efficient and faithful transcription of the full-length mirror-image 5<i>S</i>, 16<i>S</i>, and 23<i>S</i> ribosomal RNAs from enzymatically assembled long mirror-image genes. We further exploited the versatile mirror-image T7 transcription system for practical applications such as biostable mirror-image riboswitch sensor, long-term storage of unprotected kilobase-long l-RNA in water, and l-ribozyme-catalyzed l-RNA polymerization to serve as a model system for basic RNA research.
Medical subject headings
- Ribosomes
- RNA, Catalytic
- RNA, Ribosomal, 23S
- RNA, Ribosomal, 5S
- Transcription, Genetic
- DNA-Directed DNA Polymerase
- Viral Proteins