Flexible hyperspectral surface plasmon resonance microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36309515.
- Also identified by DOI 10.1038/s41467-022-34196-7 and PMC identifier 9617892.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Optical techniques for visualization and quantification of chemical and biological analytes are always highly desirable. Here we show a hyperspectral surface plasmon resonance microscopy (HSPRM) system that uses a hyperspectral microscope to analyze the selected area of SPR image produced by a prism-based spectral SPR sensor. The HSPRM system enables monochromatic and polychromatic SPR imaging and single-pixel spectral SPR sensing, as well as two-dimensional quantification of thin films with the measured resonance-wavelength images. We performed pixel-by-pixel calibration of the incident angle to remove pixel-to-pixel differences in SPR sensitivity, and demonstrated the HSPRM's capabilities by using it to quantify monolayer graphene thickness distribution, inhomogeneous protein adsorption and single-cell adhesion. The HSPRM system has a wide spectral range from 400 nm to 1000 nm, an optional field of view from 0.884 mm<sup>2</sup> to 0.003 mm<sup>2</sup> and a high lateral resolution of 1.2 μm, demonstrating an innovative breakthrough in SPR sensor technology.
Medical subject headings
- Surface Plasmon Resonance
- Graphite