A low-cost, open-source evolutionary bioreactor and its educational use.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 36317871.
- Also identified by DOI 10.7554/eLife.83067 and PMC identifier 9683782.
- Licence recorded as CC0.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolutionary biorEactor (EVE) that can be built by students with minimal engineering and programming experience. We describe how we validated EVE in a real classroom setting by evolving replicate <i>Escherichia coli</i> populations under chloramphenicol challenge, thereby enabling students to learn about bacterial growth and antibiotic resistance.
Medical subject headings
- Drug Resistance, Bacterial
- Escherichia coli Infections