An improved imaging system that corrects MS2-induced RNA destabilization.
basic_science · Level V
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- Record sourced from PubMed, PMID 36357695.
- Also identified by DOI 10.1038/s41592-022-01658-1 and PMC identifier 7613886.
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Abstract
The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.
Medical subject headings
- Capsid Proteins
- Saccharomyces cerevisiae