Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types.
basic_science · Level V
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- Record sourced from PubMed, PMID 36378956.
- Also identified by DOI 10.1126/science.abm8797 and PMC identifier 9703638.
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Abstract
Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current indicators lack the requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green and red voltage sensors, Ace-mNeon2 and VARNAM2, and their reverse response-polarity variants pAce and pAceR. Our indicators enable 0.4- to 1-kilohertz voltage recordings from >50 spiking neurons per field of view in awake mice and ~30-minute continuous imaging in flies. Using dual-polarity multiplexed imaging, we uncovered brain state-dependent antagonism between neocortical somatostatin-expressing (SST<sup>+</sup>) and vasoactive intestinal peptide-expressing (VIP<sup>+</sup>) interneurons and contributions to hippocampal field potentials from cell ensembles with distinct axonal projections. By combining three mutually compatible indicators, we performed simultaneous triple-population imaging. These approaches will empower investigations of the dynamic interplay between neuronal subclasses at single-spike resolution.
Medical subject headings
- Action Potentials
- Hippocampus
- Neurons
- Molecular Imaging
- Visual Cortex