AlphaPulldown-a python package for protein-protein interaction screens using AlphaFold-Multimer.

Yu, Dingquan; Chojnowski, Grzegorz; Rosenthal, Maria; Kosinski, Jan · Bioinformatics · 2023

basic_science · Level V

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Abstract

The artificial intelligence-based structure prediction program AlphaFold-Multimer enabled structural modelling of protein complexes with unprecedented accuracy. Increasingly, AlphaFold-Multimer is also used to discover new protein-protein interactions (PPIs). Here, we present AlphaPulldown, a Python package that streamlines PPI screens and high-throughput modelling of higher-order oligomers using AlphaFold-Multimer. It provides a convenient command-line interface, a variety of confidence scores and a graphical analysis tool. AlphaPulldown is freely available at https://www.embl-hamburg.de/AlphaPulldown. Supplementary note is available at Bioinformatics online.

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