Reconstitution of a minimal motility system based on <i>Spiroplasma</i> swimming by two bacterial actins in a synthetic minimal bacterium.

Abstract

Motility is one of the most important features of life, but its evolutionary origin remains unknown. In this study, we focused on <i>Spiroplasma</i>, commensal, or parasitic bacteria. They swim by switching the helicity of a ribbon-like cytoskeleton that comprises six proteins, each of which evolved from a nucleosidase and bacterial actin called MreB. We expressed these proteins in a synthetic, nonmotile minimal bacterium, JCVI-syn3B, whose reduced genome was computer-designed and chemically synthesized. The synthetic bacterium exhibited swimming motility with features characteristic of <i>Spiroplasma</i> swimming. Moreover, combinations of <i>Spiroplasma</i> MreB4-MreB5 and MreB1-MreB5 produced a helical cell shape and swimming. These results suggest that the swimming originated from the differentiation and coupling of bacterial actins, and we obtained a minimal system for motility of the synthetic bacterium.