Anomalies in dye-terminator DNA sequencing caused by a natural G-quadruplex.
basic_science · Level V
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- Record sourced from PubMed, PMID 36574393.
- Also identified by DOI 10.1371/journal.pone.0279423 and PMC identifier 9794070.
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Abstract
A G-rich DNA sequence from yeast that can form a non-canonical G-quadruplex structure was cloned into a plasmid vector and subjected to Sanger sequencing using dye-labeled dideoxynucleotides. Two different effects were observed. In one, presence of the G4 sequence on the template strand led to incorrect incorporation of an A residue at an internal position in the G4 sequence. In the other, the nascent strand caused attenuation of the readout coincident with synthesis of the G-rich DNA. The two effects are novel examples of disruption in DNA synthesis caused by a G4 sequence. These results provide a new example of a DNA structure that could influence genomic stability in human cells.
Medical subject headings
- G-Quadruplexes