simpleISM-A straight forward guide to upgrade from confocal to ISM.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36574395.
- Also identified by DOI 10.1371/journal.pone.0279378 and PMC identifier 9794089.
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Abstract
Resolution in a confocal laser scanning microscopes (CLSM) can be improved if the pinhole is closed. But closing the pinhole will deteriorate the signal to noise ratio (SNR). A simple technique to improve the SNR while keeping the resolution same by upgrading the system to an image scanning microscope. In this paper, we explain in detail, based on an Olympus Fluoview 300 system, how a scanning microscope can be upgraded into an image scanning microscope (ISM) using a simple camera-based detector and an Arduino Due providing a galvo driving and camera synchronization signals. We could confirm a resolution improvement as well as superconcentration and made the interesting observation of a reduced influence of laser fluctuations.
Medical subject headings
- Lasers