Solid-phase capture and profiling of open chromatin by spatial ATAC.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36604544.
- Also identified by DOI 10.1038/s41587-022-01603-9 and PMC identifier 10421738.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Current methods for epigenomic profiling are limited in their ability to obtain genome-wide information with spatial resolution. We introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially resolved epigenomics. We show that spatial ATAC enables the discovery of the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation and in human pathology.
Medical subject headings
- Chromatin
- Transposases