Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36624150.
- Also identified by DOI 10.1038/s41587-022-01595-6.
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Abstract
Here we developed an adenine transversion base editor, AYBE, for A-to-C and A-to-T transversion editing in mammalian cells by fusing an adenine base editor (ABE) with hypoxanthine excision protein N-methylpurine DNA glycosylase (MPG). We also engineered AYBE variants enabling targeted editing at genomic loci with higher transversion editing activity (up to 72% for A-to-C or A-to-T editing).
Medical subject headings
- Adenine
- DNA Glycosylases