Teasing apart the evolution of lipoprotein trafficking in gram-negative bacteria reveals a bifunctional LolA.

Abstract

The outer membrane (OM) is the defining feature of gram-negative bacteria and is an essential organelle. Accordingly, OM assembly pathways and their essential protein components are conserved throughout all gram-negative species. Lipoprotein trafficking lies at the heart of OM assembly since it supplies several different biogenesis machines with essential lipoproteins. The <i>Escherichia coli</i> Lol trafficking pathway relies on an inner membrane LolCDE transporter that transfers newly made lipoproteins to the chaperone LolA, which rapidly traffics lipoproteins across the periplasm to LolB for insertion into the OM. Strikingly, many gram-negative species (like <i>Caulobacter vibrioides</i>) do not produce LolB, yet essential lipoproteins are still trafficked to the OM. How the final step of trafficking occurs in these organisms has remained a long-standing mystery. We demonstrate that LolA from <i>C. vibrioides</i> can complement the deletion of both LolA and LolB in <i>E. coli</i>, revealing that this protein possesses both chaperone and insertion activities. Moreover, we define the region of <i>C. vibrioides</i> LolA that is responsible for its bifunctionality. This knowledge enabled us to convert <i>E. coli</i> LolA into a similarly bifunctional protein, capable of chaperone and insertion activities. We propose that a bifunctional LolA eliminates the need for LolB. Our findings provide an explanation for why some gram-negative species have retained an essential LolA yet completely lack a dedicated LolB protein.