Novel Ionizable Lipid Nanoparticles for SARS-CoV-2 Omicron mRNA Delivery.

Long, Jinrong; Yu, Changxiao; Zhang, Honglei; Cao, Yiming; Sang, Ye; Lu, Haitao; Zhang, Zhen; Wang, Xin et al. · Adv Healthc Mater · 2023

basic_science · Level V

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Abstract

mRNA-based therapy has emerged as the most promising nucleic acid therapy in the fight against COVID-19. However, a safe and efficacious systemic delivery remains a challenge for mRNA therapy. Lipid nanoparticles (LNPs) are currently widely used in mRNA delivery vehicles. Here, a series of ionizable LNPs is rationally designed. YK009-LNP is an optimal delivery platform to carry mRNA. YK009-LNP exhibits higher mRNA delivery efficiency, a more favorable biodistribution pattern, and better safety than the approved MC3-LNP. In addition, mRNA encoding severe acute respiratory syndrome coronavirus 2 Omicron receptor binding domain protein is synthesized and intramuscular administration of mice with YK009-LNP-Omicron mRNA induces a robust immune response and immune protective effect. A novel mRNA delivery vehicle with more powerful delivery efficiency and better safety than the approved LNPs is provided here.

Medical subject headings