LILAC: enhanced actin imaging with an optogenetic Lifeact.
basic_science · Level V
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- Record sourced from PubMed, PMID 36717692.
- Also identified by DOI 10.1038/s41592-022-01761-3 and PMC identifier 10986358.
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Abstract
Lifeact is a popular peptide-based label of actin filaments in live cells. We have designed an improved Lifeact variant, LILAC, that binds to actin in light using the LOV2 protein. Light control allows the user to modulate actin labeling, enabling image analysis that leverages modulation for an enhanced view of F-actin dynamics in cells. Furthermore, the tool reduces actin perturbations and cell sickness caused by Lifeact overexpression.
Medical subject headings
- Actins
- Optogenetics