Spatial tropism profiling of AAV vectors by ultrasensitive sequential FISH in tissue.

Nat Biotechnol · 2023

basic_science · Level V

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Abstract

Ultrasensitive sequential fluorescence in situ hybridization (USeqFISH) enables multiplexed detection of the expression of endogenous and exogenous genes delivered by adeno-associated virus (AAV) vectors in intact tissue. USeqFISH provides a spatial map of AAV tropism with high throughput and resolution.

Medical subject headings