Biphasic nature of lipid bilayers assembled on silica nanoparticles and evidence for an interdigitated phase.

Abstract

Functionalizing silica nanoparticles with a lipid bilayer shell is a common first step in fabricating drug delivery and biosensing devices that are further decorated with other biomolecules for a range of nanoscience applications and therapeutics. Although the molecular structure and dynamics of lipid bilayers have been thoroughly investigated on larger 100 nm-1 μm silica spheres where the lipid bilayer exhibits the typical L_<i>α</i> bilayer phase, the molecular organization of lipids assembled on mesoscale (4-100 nm diameter) nanoparticles is scarce. Here, DSC, TEM and ²H and ³¹P solid-state NMR are implemented to probe the organization of 1,2-dipalmitoyl-d₅₄-<i>glycero</i>-3-phosphocholine (DMPC-d₅₄) assembled on mesoscale silica nanoparticles illustrating a significant deviation from L_<i>α</i> bilayer structure due to the increasing curvature of mesoscale supports. A biphasic system is observed that exhibits a combination of high-curvature, non-lamellar and lamellar phases for mesoscale (<100 nm) supports with evidence of an interdigitated phase on the smallest diameter support (4 nm).