High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36823353.
- Also identified by DOI 10.1038/s41587-023-01676-0 and PMC identifier 10567548.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We profiled 189 proteins and whole transcriptome in multiple mouse tissue types with spatial CITE sequencing and then further applied the method to measure 273 proteins and transcriptome in human tissues, revealing spatially distinct germinal center reactions in tonsil and early immune activation in skin at the Coronavirus Disease 2019 mRNA vaccine injection site.
Medical subject headings
- Transcriptome
- Single-Cell Analysis