Interstrand crosslinking of homologous repair template DNA enhances gene editing in human cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36849829.
- Also identified by DOI 10.1038/s41587-022-01654-y and PMC identifier 10460463.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We describe a strategy to boost the efficiency of gene editing via homology-directed repair (HDR) by covalently modifying the template DNA with interstrand crosslinks. Crosslinked templates (xHDRTs) increase Cas9-mediated editing efficiencies by up to fivefold in K562, HEK293T, U2OS, iPS and primary T cells. Increased editing from xHDRTs is driven by events on the template molecule and requires ataxia telangiectasia and Rad3-related (ATR) kinase and components of the Fanconi anemia pathway.
Medical subject headings
- Gene Editing
- DNA