Enhanced single RNA imaging reveals dynamic gene expression in live animals.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36867026.
- Also identified by DOI 10.7554/eLife.82178 and PMC identifier 10032653.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Imaging endogenous mRNAs in live animals is technically challenging. Here, we describe an MS2-based signal amplification with the Suntag system that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1300 nt 24xMS2 into the genome for the imaging of endogenous mRNAs. Using this tool, we were able to image the activation of gene expression and the dynamics of endogenous mRNAs in the epidermis of live <i>C. elegans</i>.
Medical subject headings
- RNA
- Caenorhabditis elegans