A Multiparametric and High-Throughput Platform for Host-Virus Binding Screens.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36892970.
- Also identified by DOI 10.1021/acs.nanolett.2c04884 and PMC identifier 10176574.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Speed is key during infectious disease outbreaks. It is essential, for example, to identify critical host binding factors to pathogens as fast as possible. The complexity of host plasma membrane is often a limiting factor hindering fast and accurate determination of host binding factors as well as high-throughput screening for neutralizing antimicrobial drug targets. Here, we describe a multiparametric and high-throughput platform tackling this bottleneck and enabling fast screens for host binding factors as well as new antiviral drug targets. The sensitivity and robustness of our platform were validated by blocking SARS-CoV-2 particles with nanobodies and IgGs from human serum samples.
Medical subject headings
- SARS-CoV-2
- COVID-19