Cytotoxic CD161^-CD8⁺ T_EMRA cells contribute to the pathogenesis of systemic lupus erythematosus.

Abstract

Systemic lupus erythematosus (SLE) is a prototypical autoimmune disease affecting multiple organs and tissues with high cellular heterogeneity. CD8⁺ T cell activity is involved in the SLE pathogenesis. However, the cellular heterogeneity and the underlying mechanisms of CD8⁺ T cells in SLE remain to be identified. Single-cell RNA sequencing (scRNA-seq) of PBMCs from a SLE family pedigree (including 3 HCs and 2 SLE patients) was performed to identify the SLE-associated CD8⁺ T cell subsets. Flow cytometry analysis of a SLE cohort (including 23 HCs and 33 SLE patients), qPCR analysis of another SLE cohort (including 30 HCs and 25 SLE patients) and public scRNA-seq datasets of autoimmune diseases were employed to validate the finding. Whole-exome sequencing (WES) of this SLE family pedigree was used to investigate the genetic basis in dysregulation of CD8⁺ T cell subsets identified in this study. Co-culture experiments were performed to analyze the activity of CD8⁺ T cells. We elucidated the cellular heterogeneity of SLE and identified a new highly cytotoxic CD8⁺ T cell subset, CD161^-CD8⁺ T_EMRA cell subpopulation, which was remarkably increased in SLE patients. Meanwhile, we discovered a close correlation between mutation of DTHD1 and the abnormal accumulation of CD161^-CD8⁺ T_EMRA cells in SLE. DTHD1 interacted with MYD88 to suppress its activity in T cells and DTHD1 mutation promoted MYD88-dependent pathway and subsequently increased the proliferation and cytotoxicity of CD161^-CD8⁺ T_EMRA cells. Furthermore, the differentially expressed genes in CD161^-CD8⁺ T_EMRA cells displayed a strong out-of-sample prediction for case-control status of SLE. This study identified DTHD1-associated expansion of CD161^-CD8⁺ T_EMRA cell subpopulation is critical for SLE. Our study highlights genetic association and cellular heterogeneity of SLE pathogenesis and provides a mechanistical insight into the diagnosis and treatment of SLE. Stated in the Acknowledgements section of the manuscript.