Sampling the proteome by emerging single-molecule and mass spectrometry methods.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 36899164.
- Also identified by DOI 10.1038/s41592-023-01802-5 and PMC identifier 10044470.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Mammalian cells have about 30,000-fold more protein molecules than mRNA molecules, which has major implications in the development of proteomics technologies. We review strategies that have been helpful for counting billions of protein molecules by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and suggest that these strategies can benefit single-molecule methods, especially in mitigating the challenges of the wide dynamic range of the proteome.
Medical subject headings
- Proteome
- Specimen Handling