Two-photon fluorescence imaging and specifically biosensing of norepinephrine on a 100-ms timescale.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36918539.
- Also identified by DOI 10.1038/s41467-023-36869-3 and PMC identifier 10014876.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Norepinephrine (NE) is a key neurotransmitter in the central nervous system of organisms; however, specifically tracking the transient NE dynamics with high spatiotemporal resolution in living systems remains a great challenge. Herein, we develop a small molecular fluorescent probe that can precisely anchor on neuronal cytomembranes and specifically respond to NE on a 100-ms timescale. A unique dual acceleration mechanism of molecular-folding and water-bridging is disclosed, which boosts the reaction kinetics by ˃10<sup>5</sup> and ˃10<sup>3</sup> times, respectively. Benefiting from its excellent spatiotemporal resolution, the probe is applied to monitor NE dynamics at the single-neuron level, thereby, successfully snapshotting the fast fluctuation of NE levels at neuronal cytomembranes within 2 s. Moreover, two-photon fluorescence imaging of acute brain tissue slices reveals a close correlation between downregulated NE levels and Alzheimer's disease pathology as well as antioxidant therapy.
Medical subject headings
- Norepinephrine
- Alzheimer Disease