Solar-powered multi-organism symbiont mimic system for beyond natural synthesis of polypeptides from CO<sub>2</sub> and N<sub>2</sub>.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36921057.
- Also identified by DOI 10.1126/sciadv.adf6772 and PMC identifier 10017035.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Developing artificial symbionts beyond natural synthesis limitations would bring revolutionary contributions to agriculture, medicine, environment, etc. Here, we initiated a solar-driven multi-organism symbiont, which was assembled by the CO<sub>2</sub> fixation module of <i>Synechocystis</i> sp., N<sub>2</sub> fixation module of <i>Rhodopseudomonas palustris</i>, biofunctional polypeptides synthesis module of <i>Bacillus licheniformis</i>, and the electron transfer module of conductive cationic poly(fluorene-<i>co</i>-phenylene) derivative. The modular design broke the pathway to synthesize γ-polyglutamic acid (γ-PGA) using CO<sub>2</sub> and N<sub>2</sub>, attributing to the artificially constructed direct interspecific substance and electron transfer. So, the intracellular ATP and NADPH were enhanced by 69 and 30%, respectively, and the produced γ-PGA was enhanced by 104%. The strategy was further extended to produce a commercial antibiotic of bacitracin A. These achievements improve the selectivity and yield of functional polypeptides with one click by CO<sub>2</sub> and N<sub>2</sub>, and also provide an innovative strategy for creating photosynthetic systems on demand.
Medical subject headings
- Carbon Dioxide
- Bacillus licheniformis