A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 36989362.
- Also identified by DOI 10.1126/sciadv.adf9717 and PMC identifier 10058233.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We introduce a cost-effective, robust high-throughput-compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of ~$2.5 per sample.
Medical subject headings
- Proteomics
- Proteins