Merged magnetic resonance and light sheet microscopy of the whole mouse brain.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37068254.
- Also identified by DOI 10.1073/pnas.2218617120 and PMC identifier 10151475.
- Licence recorded as CC BY-NC-ND.
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Abstract
We have developed workflows to align 3D magnetic resonance histology (MRH) of the mouse brain with light sheet microscopy (LSM) and 3D delineations of the same specimen. We start with MRH of the brain in the skull with gradient echo and diffusion tensor imaging (DTI) at 15 μm isotropic resolution which is ~ <i>1,000 times</i> higher than that of most preclinical MRI. Connectomes are generated with superresolution tract density images of ~5 μm. Brains are cleared, stained for selected proteins, and imaged by LSM at 1.8 μm/pixel. LSM data are registered into the reference MRH space with labels derived from the ABA common coordinate framework. The result is a <b>hi</b>gh-<b>d</b>imensional <b>i</b>ntegrated <b>v</b>olum<b>e</b> with <b>r</b>egistration (<b>HiDiver</b>) with alignment precision better than 50 µm. Throughput is sufficiently high that HiDiver is being used in quantitative studies of the impact of gene variants and aging on mouse brain cytoarchitecture and connectomics.
Medical subject headings
- Diffusion Tensor Imaging
- Microscopy