Highly efficient and rapid generation of genetic variants.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37142768.
- Also identified by DOI 10.1038/s41592-023-01869-0 and PMC identifier 10158673.
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Abstract
A new mutagenesis platform enables the fast, cost-efficient and automatable production of defined multi-site sequence variants for a wide range of applications. Demonstrations of this method included the generation of SARS-CoV-2 spike gene variants, DNA fragments for large-scale genome engineering, and adeno-associated virus 2 (AAV2) cap genes with improved packaging capacity.
Medical subject headings
- High-Throughput Nucleotide Sequencing