Genetic engineering meets hematopoietic stem cell biology for next-generation gene therapy.

Ferrari, Samuele; Valeri, Erika; Conti, Anastasia; Scala, Serena; Aprile, Annamaria; Di Micco, Raffaella; Kajaste-Rudnitski, Anna; Montini, Eugenio et al. · Cell Stem Cell · 2023

review · Level V

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Abstract

The growing clinical success of hematopoietic stem/progenitor cell (HSPC) gene therapy (GT) relies on the development of viral vectors as portable "Trojan horses" for safe and efficient gene transfer. The recent advent of novel technologies enabling site-specific gene editing is broadening the scope and means of GT, paving the way to more precise genetic engineering and expanding the spectrum of diseases amenable to HSPC-GT. Here, we provide an overview of state-of-the-art and prospective developments of the HSPC-GT field, highlighting how advances in biological characterization and manipulation of HSPCs will enable the design of the next generation of these transforming therapeutics.

Medical subject headings