Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei.

Zhao, Jie; Zhang, Meng; Hui, Wenyan; Zhang, Yue; Wang, Jing; Wang, Shaojing; Kwok, Lai-Yu; Kong, Jian et al. · Nat Commun · 2023

basic_science · Level V

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Abstract

Lacticaseibacillus paracasei is an economically important bacterial species, used in the food industry and as a probiotic. Here, we investigate the roles of N6-methyladenine (6mA) modification in L. paracasei using multi-omics and high-throughput chromosome conformation capture (Hi-C) analyses. The distribution of 6mA-modified sites varies across the genomes of 28 strains, and appears to be enriched near genes involved in carbohydrate metabolism. A pglX mutant, defective in 6mA modification, shows transcriptomic alterations but only modest changes in growth and genomic spatial organization.

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