High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37156843.
- Also identified by DOI 10.1038/s41592-023-01866-3 and PMC identifier 10250191.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5-10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications, removing a key obstacle for connectomic analyses at the mammalian whole-brain level.
Medical subject headings
- Connectome