Targeted knock-ins with pseudovirus for the stable expression of large transgenes.
basic_science · Level V
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- Record sourced from PubMed, PMID 37173433.
- Also identified by DOI 10.1038/s41551-023-01044-y and PMC identifier 10177726.
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Abstract
We engineered integrase-deficient lentiviruses to act as vectors for the delivery of large gene knock-ins via homology-directed repair. This technology enables the non-cytotoxic, targeted insertion of difficult-to-express transgenes into genomic loci that are essential to cell survival, thereby overcoming the gene silencing that otherwise limits primary immune cell engineering.