Rapid and precise genome engineering in a naturally short-lived vertebrate.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37191291.
- Also identified by DOI 10.7554/eLife.80639 and PMC identifier 10188113.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here, we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application to precisely insert fluorescent reporters of different sizes at various genomic loci in order to drive cell-type- and tissue-specific expression. This knock-in method should allow the establishment of humanized disease models and the development of cell-type-specific molecular probes for studying complex vertebrate biology.
Medical subject headings
- Vertebrates
- Fundulidae