Injectable Antibacterial Hydrogel with Asiaticoside-Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn-Infected Wounds.

Abstract

Post-injury infection and wound healing are recurrent daily life problems. Therefore, the necessity of developing a biomaterial with antibacterial and wound-healing properties is paramount. Based on the special porous structure of hydrogel, this work modifies recombinant collagen and quaternary ammonium chitosan and fused them with silver nanoparticles (Ag@mental-organic framework (Ag@MOF)) with antibacterial properties, and asiaticoside-loaded liposomes (Lip@AS) with anti-inflammatory/vascularization effects to form the rColMA/QCSG/LIP@AS/Ag@MOF (RQLAg) hydrogel. The prepared hydrogel possesses good sustainable release capabilities of Ag⁺ and AS and exhibits concentration-dependent swelling properties, pore size, and compressive strength. Cellular experiments show that the hydrogel exhibits good cell compatibility and promote cell migration, angiogenesis, and M1 macrophage polarization. Additionally, the hydrogels exhibit excellent antibacterial activity against Escherichia coli and Staphylococcus aureus in vitro. In vivo, Sprague Dawley rats burn-wound infection model showed that the RQLAg hydrogel could efficiently promote wound healing and has stronger healing promoting abilities than those of Aquacel Ag. In summary, the RQLAg hydrogel is expected to be an excellent material for accelerating open wound healing and preventing bacterial infections.