GIL: a python package for designing custom indexing primers.

Mateyko, Nicholas; Tariq, Omar; Chen, Xinyi E; Cheney, Will; Salaudeen, Asfar Lathif; Luthra, Ishika; Nikpour, Najmeh; Rafi, Abdul Muntakim et al. · Bioinformatics · 2023

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Abstract

Generate Indexes for Libraries (GIL) is a software tool for generating primers to be used in the production of multiplexed sequencing libraries. GIL can be customized in numerous ways to meet user specifications, including length, sequencing modality, color balancing, and compatibility with existing primers, and produces ordering and demultiplexing-ready outputs. GIL is written in Python and is freely available on GitHub under the MIT license: https://github.com/de-Boer-Lab/GIL and can be accessed as a web-application implemented in Streamlit at https://dbl-gil.streamlitapp.com.

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