Simultaneous sulfide and methane oxidation by an extremophile.

Abstract

Hydrogen sulfide (H₂S) and methane (CH₄) are produced in anoxic environments through sulfate reduction and organic matter decomposition. Both gases diffuse upwards into oxic zones where aerobic methanotrophs mitigate CH₄ emissions by oxidizing this potent greenhouse gas. Although methanotrophs in myriad environments encounter toxic H₂S, it is virtually unknown how they are affected. Here, through extensive chemostat culturing we show that a single microorganism can oxidize CH₄ and H₂S simultaneously at equally high rates. By oxidizing H₂S to elemental sulfur, the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV alleviates the inhibitory effects of H₂S on methanotrophy. Strain SolV adapts to increasing H₂S by expressing a sulfide-insensitive ba₃-type terminal oxidase and grows as chemolithoautotroph using H₂S as sole energy source. Genomic surveys revealed putative sulfide-oxidizing enzymes in numerous methanotrophs, suggesting that H₂S oxidation is much more widespread in methanotrophs than previously assumed, enabling them to connect carbon and sulfur cycles in novel ways.