Simultaneous profiling of spatial gene expression and chromatin accessibility during mouse brain development.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37231265.
- Also identified by DOI 10.1038/s41592-023-01884-1.
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Abstract
The brain is a complex tissue whose function relies on coordinated anatomical and molecular features. However, the molecular annotation of the spatial organization of the brain is currently insufficient. Here, we describe microfluidic indexing-based spatial assay for transposase-accessible chromatin and RNA-sequencing (MISAR-seq), a method for spatially resolved joint profiling of chromatin accessibility and gene expression. By applying MISAR-seq to the developing mouse brain, we study tissue organization and spatiotemporal regulatory logics during mouse brain development.
Medical subject headings
- Chromatin
- High-Throughput Nucleotide Sequencing