New measures of anisotropy of cryo-EM maps.
basic_science · Level V
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- Record sourced from PubMed, PMID 37248387.
- Also identified by DOI 10.1038/s41592-023-01874-3.
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Abstract
We propose two new measures of resolution anisotropy for cryogenic electron microscopy maps: Fourier shell occupancy (FSO), and the Bingham test (BT). FSO varies from 1 to 0, with 1 representing perfect isotropy, and lower values indicating increasing anisotropy. The threshold FSO = 0.5 occurs at Fourier shell correlation resolution. BT is a hypothesis test that complements the FSO to ensure the existence of anisotropy. FSO and BT allow visualization of resolution anisotropy. We illustrate their use with different experimental cryogenic electron microscopy maps.
Medical subject headings
- Cryoelectron Microscopy