High-throughput RNA isoform sequencing using programmed cDNA concatenation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37291427.
- Also identified by DOI 10.1038/s41587-023-01815-7 and PMC identifier 12236355.
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Abstract
Full-length RNA-sequencing methods using long-read technologies can capture complete transcript isoforms, but their throughput is limited. We introduce multiplexed arrays isoform sequencing (MAS-ISO-seq), a technique for programmably concatenating complementary DNAs (cDNAs) into molecules optimal for long-read sequencing, increasing the throughput >15-fold to nearly 40 million cDNA reads per run on the Sequel IIe sequencer. When applied to single-cell RNA sequencing of tumor-infiltrating T cells, MAS-ISO-seq demonstrated a 12- to 32-fold increase in the discovery of differentially spliced genes.
Medical subject headings
- RNA Isoforms
- High-Throughput Nucleotide Sequencing